Methionine transfer RNAF: the initiator transfer RNA for hemoglobin biosynthesis.
نویسندگان
چکیده
This paper presents evidence suggesting that methionyltRNAp is the initiator tRNA for hemoglobin biosynthesis. Our experimental approach was as follows. If the mechanism of mammalian protein synthesis is similar to the mechanism in bacteria, then a tRNA species should be present in the cytoplasm of reticulocytes which has the unique property of being recognized only by its own "initiation" factors. This
منابع مشابه
Initiator methionine transfer ribonucleic acid from wheat embryo. Purification, properties, and partial nucleotide sequences.
Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...
متن کاملMethionine transfer ribonucleic acids of avian myeloblastosis virus.
Chick-embryo cells contain four isoaccepting species of methionine transfer RNA (I-IV). One species (I) is the initiator, tRNA(f) (Met), and the others (II, III, and IV) are the donors of internal methionyl residues (tRNA(m) (Met)). Over 85% of the tRNA(Met) in purified avian myeloblastosis virus consists of one tRNA(m) (Met) species, which resembles host-cell tRNA(Met) IV with respect to chrom...
متن کاملMethionine Biosynthesis in Staphylococcus aureus Is Tightly Controlled by a Hierarchical Network Involving an Initiator tRNA-Specific T-box Riboswitch
In line with the key role of methionine in protein biosynthesis initiation and many cellular processes most microorganisms have evolved mechanisms to synthesize methionine de novo. Here we demonstrate that, in the bacterial pathogen Staphylococcus aureus, a rare combination of stringent response-controlled CodY activity, T-box riboswitch and mRNA decay mechanisms regulate the synthesis and stab...
متن کاملTwo eukaryotic initiation factors (IF-I and IF-II) of protein synthesis that are required to form an initiation complex with rabbit reticulocyte ribosomes.
The formation of an initiation complex with rabbit reticulocyte ribosomes and certain templates is dependent upon the action of two initiation factors (IF-I and IF-II) of protein synthesis isolated from 0.5 M KCl extracts of rabbit reticulocyte polysomes. IF-I mediates the GTP-dependent, but template-independent, binding of the cytoplasmic initiator transfer RNA (rabbit liver) to a 40S ribosoma...
متن کاملCharacterization of two species of methionine transfer ribonucleic acid from bakers' yeast.
The biochemical properties of two species of methionine transfer ribonucleic acid from bakers’ yeast, separated by diethylaminoethyl Sephadex column chromatography, have been studied. Each species of the two methionine tRNAs, designated methionine tRNA I and methionine tRNA II, respectively, has been purified by benzoylated DEAEcellulose column chromatography. Methionine tRNA I can be esterifie...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cancer research
دوره 31 5 شماره
صفحات -
تاریخ انتشار 1971